Antifungal Substances Produced by B. subtilis Strain W3.15 Inhibit the Fusarium oxysporum and Trigger Cellular Damage

Soybean Fusarium wilt and root rot disease caused by a necrotrophic ascomycete pathogen, F. oxysporum, triggered severe damage to the plant tissues organs impacted heavy losses. Biocontrol agents, Bacillus subtilis, were commonly used produce broad spectrum of antifungal substances gradually in biocontrol studies for management. Investigation determination inhibiting mechanism substance produced B. subtilis on oxysporum should be done protect soybean plant. This study revealed that basal nutrient broth (NB) gives best activity. The stationary phase bacterial growth curve was obtained two days cultivation showed maximum activity against oxysporum. Ethyl acetate (EA) extraction supernatant generated crude EA extract, which half inhibition (IC50) at 306.42 µg/ml from dose-response regression curve. Post-treatment mycelia with extract demonstrated as hyphal deformation followed malondialdehyde (MDA) accumulation. Furthermore, cellular leakage fungal cells may compounds strain W3.15 occurred. Last, related predicted epicatechin benzophenone LC-MS/MS analysis extract. Accordingly, agent promises strong potency biofungicide development.